Monitoring pH and whey protein digestion by TD-NMR and MRI in a novel semi-dynamic in vitro gastric simulator (MR-GAS)

Gastric digestion is crucial for protein breakdown. Magnetic resonance techniques have a great deal of potential but remain underexplored with regard to their application in the study food via MRI-markers, such as transverse (R2) and longitudinal (R1) relaxation rates. R2 has been used monitor gastric whey gels, only static vitro model. It essential investigate whether rates can be valid measures under dynamic circumstances. We developed novel MRI-compatible semi-dynamic simulator (MR-GAS) that includes controlled secretion, emptying mixing at body temperature. PH hydrolysis were measured during gel MR-GAS. R1 supernatant by time-domain nuclear magnetic (TD-NMR). The stomach chamber MR-GAS was also scanned MRI measure R1. For TD-NMR, 99% variance 96% could explained function concentration [H+]. MRI, variances 60% From these analysis, obtained equations enabled prediction pH normalised root mean squared deviation predictions 0.15 (NMR) 0.18 (MRI), 0.12 0.29 (MRI). In conclusion, model may clinical circumstances, measuring These results underscore nutrients changes future vivo studies.